Table 4.
Major clusters of T. brucei specific proteins identified in this study
| Protein family | UniProt ID | Protein name | Functional roles |
|---|---|---|---|
| Variant/invariant surface glycoproteins (VSG/ISG) | Q26842 | VSG | VSGs/ISGs are activated in the SGs; involved in immune evasion/resistance |
| M4TB38 | VSG 1228 | ||
| M4SU87 | VSG 725 | ||
| Q57VX7 | 75 kDa ISG | ||
| B2ZWC6 | ISG | ||
| Retrotransposon hot spot protein (RHS) multigene family | Q8T9M3 | RHS1a | RHSs are diverse and potentially rapidly evolving nuclear and perinuclear proteins in T. brucei. RHSs are located in the polymorphic subtelomic regions and may therefore confer selective advantages for the evasion of host immune responses through antigenic variations |
| Q8T9M7 | RHS2a | ||
| Q8WPS8 | H25N7.12 protein (RHS4) | ||
| Q8T9M4 | RHS6a | ||
| D0A6L8 | Putative RHS | ||
| D7SGA2 | RHS4 | ||
| Q584N8 | Putative RHS | ||
| Q585G9 | RHS5a | ||
| Kinetoplastid calpain/small kinetoplastid calpain-related protein (CALP/SKCRP) protein family | Q4GZ11 | CALP1.1 | CALPs are well-conserved and ubiquitously expressed in tissue-specific isoforms. They are involved in virulence and various physiological (cytoskeleton rearrangement, proliferation, cellular differentiation, interaction with host structures) |
| C9ZIE8 | CALP1.2 | ||
| Q57WJ7 | CALP5.5 V | ||
| C9ZT01 | CALP7.1 | ||
| Q387E1 | CALP11.6 | ||
| Q4GZ06 | SKCRP1.4 | ||
| C9ZIF2 | SKCRP1.5 | ||
| C9ZIF6 | SKCRP1.7 | ||
| Paraflagellar/paraxial rod (PRF) protein family | C9ZVV0 | 69 kDa PFR-A | 69-kDa and 73-kDa proteins are the major structural components of T. brucei flagellar; Important for parasite motility |
| C9ZLC1 | 73 kDa PFR-C | ||
| Peroxiredoxin alkyl hydroperoxide reductase C (AhpC)-type family | C9ZL57 | Tryparedoxin | TXN/TXNPx are highly abundant in all life stages of T. brucei; Important in trypanosome metabolism (nucleotide synthesis); and oxidative defence (detoxification of hyperoxides) |
| C9ZUX7 | Tryparedoxin peroxidase | ||
| C9ZXT5 | Tryparedoxin peroxidase | ||
| Bloodstream stage alanine-rich protein (BARP) | C9ZZP8 | BARP | BARPs are GPI-anchored proteins, which are important for cytokinesis; BARPs are proposed to form stage-specific coat for epimastigote forms of T. brucei |
| O60946 | BARP | ||
| Q38CW0 | BARP | ||
| Q38CW1 | BARP | ||
| C9ZZQ0 | BARP | ||
| Molecular chaperones; heat shock proteins (Hsp) family | C9ZL02 | Putative Hsp20 | Molecular chaperones are central players in various physiological processes such as protein folding and in maintenance of cellular homeostasis/survival under optimal growth conditions |
| D0A349 | Mitochondrial Hsp60 | ||
| C9ZR44 | Mitochondrial Hsp70 | ||
| Q383E5 | Hsp70 | ||
| D0A4N5 | Hsp83 | ||
| D0A590 | Putative Hsp | ||
| Membrane transporters | Q388Z2 | Plasma membrane ATPase (PMA1) | Involved in salvage of nutrients and other metabolites from the host |
| C9ZK10 | ATP synthase subunit beta (ATP5B) | ||
| Q581D7 | Putative adenosine transporter 1 (ENT1) | ||
| Ribosomal proteins (RPs) | D0A7E1 | RPS5 | Involved in the regulation of protein translation |
| C9ZXI9 | RPS7 | ||
| O76223 | RPS12 | ||
| C9ZRH0 | RPS14 | ||
| D0A2S1 | RPS18 | ||
| C9ZZX2 | RPL10a | ||
| C9ZYV4 | RPL23 |
Functional roles were inferred from available literature. Only a selection of variant/invariant surface glycoproteins (VSGs/ISGs) is shown in this table (see full list of the isoforms and/or variants in Additional file 1: Table S3)