Fig. 2.

Gtpbp2 associates with components of the β-catenin destruction complex. a HA-Gtpbp2 was coexpressed in Hek293t cells with empty vector (Myc), Myc-Axin or Myc-Gsk3b and lysates were immunoprecipitated with anti-myc antibody followed western blot assay to detect precipitated Myc-tagged proteins and co-precipitated HA-Gtpbp2. The expression HA-Gtpbp2 in cells was confirmed by western blot on cell lysates. b mRNAs encoding HA-Gtpbp2 were injected into two-cell Xenopus embryos along with mRNAs encoding either the Myc tag, Myc-Axin, Myc-Gsk3b, or Myc-Gsk3b, as indicated. Embryos were lysed at gastrula stage 11, and were immunoprecipitated with anti-myc antibody followed western blot assay to detect precipitated Myc-tagged proteins and co-precipitated HA-Gtpbp2. HA-Gtpbp2 expression in cells was evaluated by western blot on cell lysates. c mCherry-Gtpbp2 relocalizes to GFP-Dvl2 containing granules when co-expressed in Xenopus animal caps (scored at late blastula, stage 9). mCherry-Gtpbp2 distribution is diffuse in cells lacking GFP-Dsh