Fig. 4.

Gtpbp2 epistasis experiments suggest Gtpbp2 works at the level of Axin turnover. a-d mRNAs encoding activators of the Wnt pathway, including a wnt8, b disheveled (dvl2), c kinase-dead Gsk3b (dnGsk3) or d phospho-resistant ß-catenin (ptbcat) respectively were coinjected with 40 ng control or Gtpbp2 morpholino into the animal pole of two cell Xenopus embryos. Animal caps were excised at blastula stage 8, and the levels of siamois transcript were measured using qPCR at early gastrula stage 10.5 and shown as mean ± s.e.m of n = 3. The Gtpbp2 morpholino blocks induction from wnt8, disheveled, or dominant negative dngsk3 (a-c), but not from a phospho-resistant β-catenin (ptbcat) (d)