Fig.3.

Effect of heat and LA1011 or LA1044 treatment on Hsp70, Hsp27 and Hsp40 levels in SH-SY5Y human neuroblastoma cell line measured by western blotting (A, B) and quantitative densitometric analysis (C, D). Cells were untreated or treated with LA1011 (5μM) or LA1044 (40μM) at 37°C or 42°C. Box plots represent quantitative data normalized to GAPDH. All data represent the mean±SD (n = 6) and p < 0.05 was considered statistically significant. HS = heat shock at 42°C, 1 h. ^*p < 0.05, when data are compared to LA compound (–), HS (–) sample. ^#p < 0.05, when data are compared to LA compound (–), HS (+) sample. One way ANOVA followed by a Tukey post hoc test was used for statistical comparisons.