Fig. 1.

Intracellular growth of clpP-deficient L. pneumophila strain Xp02 in J774A.1 was impaired. J774A.1 cells were seeded in 24-well plates and infected with L. pneumophila at an MOI of 1. At each time point, cells were lysed and the CFU was determined by plating dilutions onto BCYE plates. The intracellular growth kinetics of Lp02 (●), clpP mutant (■), clpP complemented strain (▲), and dotA mutant Lp03 (X) were shown. Points indicate mean values and error bars indicate standard deviations of three independent experiments