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. 2016 Aug 2;16:174. doi: 10.1186/s12866-016-0790-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The L. pneumophila clpP mutant Xp02 was degraded within 3 h of phagocytosis. J774A.1 cells were seeded in 24-well plates and infected with L. pneumophila at an MOI of 1. After 3 h, the survival of bacteria was determined by plating dilutions onto BCYE plates and calculating the numbers of CFU of L. pneumophila lysed from infected J774A.1. *, p < 0.05; **, p < 0.01. The phagocytosis assay was carried out in triplicate. Shown are the averages and standard deviations of three independent counts