Fig. 5.

The clpP-deficient L. pneumophila still has a functional T4BSS. Contact-dependent cytotoxicity of the wild-type strain Lp02, the clpP mutant Xp02, the complemented strain Xp02c and the dotA mutant Lp03. Infection was performed at an MOI of 50 and 100, and cell permeabilization was detected by the release of the intracellular enzyme lactate dehydrogenase (LDH). Shown are the averages and standard deviations of three independent experiments, each performed in triplicate (duplicate for cytotoxicity assays). And there is no statistical difference between the strains