Table 2.
Plasmids used in this study
| Plasmids | Characteristics | Reference |
|---|---|---|
| pBRDX | Suicide delivery vector, rdxA sacB Cm | [54] |
| pBRΔclpP | pBRDX::clpP for clpP deletion | This study |
| pJB908 | Insert thy gene, mutate mob into pkB5 | [4] |
| pBC(gfp)Pmip | ColE1 ori Cm Pmip gfpmut2 | [59] |
| pZL01 | Insert SacI/PstI fragment encoding GFP, Pmip into pJB908 | This study |
| pXL901 | Complementation plasmid, derived from pZL01, with gfpmut2 changed for clpP | This study |
| pTLpflaG | Insert BamHI/SphI fragment encoding GFP, flaA promoter into pTLP6 | [57] |
| pGB908 | Insert XbaI/SphI fragment of gfpmut3 into pJB908 | This study |
| pGB908X* | Insert 9 icm promoters of operon areas into pGB908 | This study |
| pCyaSidJ | Insert EcoRI/SalI fragment of CyaA catalytic domain and SidJ into pKB5 | [34, 50] |
The X* represents the numbers of the plasmids or bacterial strains containing the icm promoter-gfp fusions: 1. icmTS, 2. icmR, 3. icmQ, 4. icmPO, 5. icmMLKEGCD, 6. icmJB, 7. icmHF, 8. icmWX, 9. icmV