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. 2016 Aug 2;6:30550. doi: 10.1038/srep30550

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Minimum intensity projections of a representative neuron showing the spatiotemporal intensity distribution of cell-impermeant Alexa Fluor 568 hydrazide (AFH) after compression at strain rates of 10 s⁻¹ (top) and 75 s⁻¹ (bottom). Scale bar, 20 µm. (b) Mean fluorescence intensity within the cell boundary (inset) for (solid triangle), (solid circle), a positive control (long dash) with Triton X-100, and a negative control (short dash) without compressive loading. Scale bar, 20 µm. (c) AFH influx time (mean ± standard deviation), or peak rise in internal fluorescence, for (solid white; n = 16 cells, N = 5 experiments) and (solid gray; n = 23, N = 8), and cellular death time for (hatched white; n = 19, N = 8) and (hatched gray; n = 22, N = 8). No significant difference was found via t-test comparison.

Inline graphic — (a) Minimum intensity projections of a representative neuron showing the spatiotemporal intensity distribution of cell-impermeant Alexa Fluor 568 hydrazide (AFH) after compression at strain rates of 10 s⁻¹ (top) and 75 s⁻¹ (bottom). Scale bar, 20 µm. (b) Mean fluorescence intensity within the cell boundary (inset) for (solid triangle), (solid circle), a positive control (long dash) with Triton X-100, and a negative control (short dash) without compressive loading. Scale bar, 20 µm. (c) AFH influx time (mean ± standard deviation), or peak rise in internal fluorescence, for (solid white; n = 16 cells, N = 5 experiments) and (solid gray; n = 23, N = 8), and cellular death time for (hatched white; n = 19, N = 8) and (hatched gray; n = 22, N = 8). No significant difference was found via t-test comparison.