Figure 2. Assignments of the ¹H-NMR signals of a representative 500 MHz 1D-CPMG ¹H-NMR average spectrum of a healthy serum sample measured at 310 K.

A, full spectrum (δ 5.50–0.5 ppm) and magnification of aromatic region (δ 9.00–6.50 ppm). Peak assignments: 0, unidentified; 1, cholesterol; 2, lipids (–CH₃) (mainly LDL/VLDL); 3, leucine; 4, valine; 5, 3-hydroxybutyrate; 6, lipids (CH₂)n (mainlyLDL/VLDL); 7, lactate; 8, alanine; 9, adipicacid; 10, arginine; 11, lysine; 12, acetate; 13, lipids (CH₂–C=C); 14, acetyl signals from glycoproteins; 15, glutamine; 16, lipids (CH₂–CO); 17, citrate; 18, lipids (CH=CH–CH₂–CH=CH–); 19, Albumin lysyl; 20, creatine; 21, choline; 22, Trimethylamine N-oxide; 23, Proline; 24, glucose; 25, glycerol; 26, Myo-inositol; 27, creatinine; 28, threonine; 29, β-glucose; 30, glycerol of lipids; 31, α-glucose; 32, lipids (–CH=CH–); 33, tyrosine; 34, phenylalanine; 35, histidine; 36, 1-methylhistidine; 37, formate.