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. 2016 Aug;148(2):133–145. doi: 10.1085/jgp.201611614

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© 2016 Gosselin-Badaroudine et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Ionic selectivity of the wild-type AmCa_V4 channel. (A) Representative current traces recorded in response to a −10-mV test pulse in extracellular solutions containing divalent ions. (B) Representative current traces recorded in response to a −10 mV test pulse in the extracellular solutions containing monovalent ions. (C) The relative permeability of the ions was calculated by dividing the peak current in a given solution by the peak current in the Ca²⁺ solution (n = 3). The asterisks designate values that were deemed statistically different from the relative permeation of Ca²⁺ determined using a one-way ANOVA (*, P < 0.05; ***, P < 0.001). The number signs designate means that were deemed statistically different from 0 (no measurable current) determined using a t test (#, P < 0.05; ###, P < 0.001). All oocytes included in these series of experiments were injected with 50 nl EGTA chelating solution prior recordings. Error bars represent SEM.