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| Ringer | 0 Na+ | 0 K+ | 0 Ca2+ | 0 Mg2+ | |
|---|---|---|---|---|---|
| mM | mM | mM | mM | mM | |
| NaCl | 116 | 0 | 116 | 116 | 116 |
| KCl | 2 | 2 | 0 | 2 | 2 |
| CaCl2 | 1.8 | 1.8 | 1.8 | 0 | 1.8 |
| MgCl2 | 2 | 2 | 2 | 2 | 0 |
| HEPES | 5 | 5 | 5 | 5 | 5 |
| Choline-Cl− | 0 | 116 | 2 | 1.8 | 2 |
The pH was adjusted to 7.4 using CsOH. The osmolarity of all solutions was 245–260 mosmol.
