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. 2016 Aug;148(2):97–118. doi: 10.1085/jgp.201611619

Table 5. Changes in HV1 properties in S3 (166–188) and S3–S4 linker (189–196) mutants versus WT channels .

Mutant Species Expr. system I? τ act τ tail ΔV thr ΔpH slope Selectivity Other Reference
E164A/E171A hHV1 HEK wc Yes −7 Ramsey et al., 2010
H167N/H168V/K169N hHV1 HEK wc Yes −13 44 Ramsey et al., 2010
E171A/D174A hHV1 HEK wc Yes −116 39 Ramsey et al., 2010
E171A hHV1 Vesicle flux Δ H+ fluxa Letts, 2014
D174A hHV1 HEK wc Yes −111 38 Ramsey et al., 2010
D174A hHV1 Vesicle flux Δ H+ fluxa Letts, 2014
D174N hHV1 HEK wc Yes −142 36 Ramsey et al., 2010
D174H hHV1 HEK wc Yes −136 37 Ramsey et al., 2010
D174E hHV1 HEK wc Yes −52 46 Ramsey et al., 2010
D174A CiHV1 [D222A] Xenopus TEVC Yes −111 Chamberlin et al., 2014
D174C/R205C CiHV1 [D222C/R255C] Xenopus TEVC Yes −95 Chamberlin et al., 2014
D174C/R208C CiHV1 [D222C/R258C] Xenopus TEVC Yes 48 Chamberlin et al., 2014
V178A hHV1 Xenopus i-o Yes −27 Hong et al., 2014
D112V/V178D hHV1 COS/HEK wc No Morgan et al., 2013
S181A hHV1 HEK wc Yes 18 46 Ramsey et al., 2010
S181A hHV1 Xenopus i-o Yes 0 Hong et al., 2014
D112V/S181D hHV1 COS/HEK wc No Morgan et al., 2013
F182A hHV1 Xenopus i-o Yes −9 Hong et al., 2014
D185A hHV1 HEK wc Yes 58 47 Ramsey et al., 2010
D185M hHV1 COS/HEK wc Yes H+ Musset et al., 2011
D185V hHV1 COS/HEK wc Yes 20b 43b H+ Musset et al., 2011
D185A hHV1 COS/HEK wc Yes 42b 40b H+ Musset et al., 2011
D185A hHV1 Vesicle flux nc H+ flux Letts, 2014
D185N hHV1 COS/HEK wc Yes 36b 47b H+ Musset et al., 2011
D185C CiHV1 [D233C] Xenopus TEVC Yes 76 Chamberlin et al., 2014
E185C/R208C CiHV1 [D233C/R258C] Xenopus TEVC Yes 4 Chamberlin et al., 2014
E192A/E196A hHV1 HEK wc Yes 13 Ramsey et al., 2010
H193A hHV1 HEK wc Yes H+ 39 × Kd Zn2+ Ramsey et al., 2006
H193A hHV1 Vesicle flux nc H+ flux Letts, 2014
H140A/H193A hHV1 HEK wc Yes −12 46 H+ 2,000 × Kd Zn2+ Ramsey et al., 2006

That numerical entries are shown does not imply that any given change was significant. Italicized mutant entries from nonhuman species show the hHV1 equivalent. In the Expression system column: HEK, HEK-293, HEK-293T, tsA, or HM1; COS, COS-7; Xenopus, Xenopus laevis oocyte; wc, whole cell; i-o, inside-out patch configuration; TEVC, two-electrode voltage clamp. Blank entries indicate that the parameter was not examined. nc, measured, but no change. Parameters are given relative to WT in each study. For I?, yes means currents are detectable. Time constants are ratios of mutant/WT. The ΔVthreshold value is the change in absolute position of the gHV relationship versus WT. The ΔpH slope is the slope in millivolts of the relationship between Vthreshold (or other parameters reflecting the absolute position of the gHV relationship) versus Vrev or EH (which are not identical; see section Table entries defined).

a

Normal initial H+ flux followed by recovery ascribed to leak induced in vesicles.

b

Previously unpublished, analyzed from data for Musset et al. (2011).