Table 5. Changes in HV1 properties in S3 (166–188) and S3–S4 linker (189–196) mutants versus WT channels .
Mutant | Species | Expr. system | I? | τ act | τ tail | ΔV thr | ΔpH slope | Selectivity | Other | Reference |
---|---|---|---|---|---|---|---|---|---|---|
E164A/E171A | hHV1 | HEK wc | Yes | −7 | Ramsey et al., 2010 | |||||
H167N/H168V/K169N | hHV1 | HEK wc | Yes | −13 | 44 | Ramsey et al., 2010 | ||||
E171A/D174A | hHV1 | HEK wc | Yes | −116 | 39 | Ramsey et al., 2010 | ||||
E171A | hHV1 | Vesicle flux | Δ H+ fluxa | Letts, 2014 | ||||||
D174A | hHV1 | HEK wc | Yes | −111 | 38 | Ramsey et al., 2010 | ||||
D174A | hHV1 | Vesicle flux | Δ H+ fluxa | Letts, 2014 | ||||||
D174N | hHV1 | HEK wc | Yes | −142 | 36 | Ramsey et al., 2010 | ||||
D174H | hHV1 | HEK wc | Yes | −136 | 37 | Ramsey et al., 2010 | ||||
D174E | hHV1 | HEK wc | Yes | −52 | 46 | Ramsey et al., 2010 | ||||
D174A | CiHV1 [D222A] | Xenopus TEVC | Yes | −111 | Chamberlin et al., 2014 | |||||
D174C/R205C | CiHV1 [D222C/R255C] | Xenopus TEVC | Yes | −95 | Chamberlin et al., 2014 | |||||
D174C/R208C | CiHV1 [D222C/R258C] | Xenopus TEVC | Yes | 48 | Chamberlin et al., 2014 | |||||
V178A | hHV1 | Xenopus i-o | Yes | −27 | Hong et al., 2014 | |||||
D112V/V178D | hHV1 | COS/HEK wc | No | Morgan et al., 2013 | ||||||
S181A | hHV1 | HEK wc | Yes | 18 | 46 | Ramsey et al., 2010 | ||||
S181A | hHV1 | Xenopus i-o | Yes | 0 | Hong et al., 2014 | |||||
D112V/S181D | hHV1 | COS/HEK wc | No | Morgan et al., 2013 | ||||||
F182A | hHV1 | Xenopus i-o | Yes | −9 | Hong et al., 2014 | |||||
D185A | hHV1 | HEK wc | Yes | 58 | 47 | Ramsey et al., 2010 | ||||
D185M | hHV1 | COS/HEK wc | Yes | H+ | Musset et al., 2011 | |||||
D185V | hHV1 | COS/HEK wc | Yes | 20b | 43b | H+ | Musset et al., 2011 | |||
D185A | hHV1 | COS/HEK wc | Yes | 42b | 40b | H+ | Musset et al., 2011 | |||
D185A | hHV1 | Vesicle flux | nc H+ flux | Letts, 2014 | ||||||
D185N | hHV1 | COS/HEK wc | Yes | 36b | 47b | H+ | Musset et al., 2011 | |||
D185C | CiHV1 [D233C] | Xenopus TEVC | Yes | 76 | Chamberlin et al., 2014 | |||||
E185C/R208C | CiHV1 [D233C/R258C] | Xenopus TEVC | Yes | 4 | Chamberlin et al., 2014 | |||||
E192A/E196A | hHV1 | HEK wc | Yes | 13 | Ramsey et al., 2010 | |||||
H193A | hHV1 | HEK wc | Yes | H+ | 39 × Kd Zn2+ | Ramsey et al., 2006 | ||||
H193A | hHV1 | Vesicle flux | nc H+ flux | Letts, 2014 | ||||||
H140A/H193A | hHV1 | HEK wc | Yes | −12 | 46 | H+ | 2,000 × Kd Zn2+ | Ramsey et al., 2006 |
That numerical entries are shown does not imply that any given change was significant. Italicized mutant entries from nonhuman species show the hHV1 equivalent. In the Expression system column: HEK, HEK-293, HEK-293T, tsA, or HM1; COS, COS-7; Xenopus, Xenopus laevis oocyte; wc, whole cell; i-o, inside-out patch configuration; TEVC, two-electrode voltage clamp. Blank entries indicate that the parameter was not examined. nc, measured, but no change. Parameters are given relative to WT in each study. For I?, yes means currents are detectable. Time constants are ratios of mutant/WT. The ΔVthreshold value is the change in absolute position of the gH–V relationship versus WT. The ΔpH slope is the slope in millivolts of the relationship between Vthreshold (or other parameters reflecting the absolute position of the gH–V relationship) versus Vrev or EH (which are not identical; see section Table entries defined).
Normal initial H+ flux followed by recovery ascribed to leak induced in vesicles.
Previously unpublished, analyzed from data for Musset et al. (2011).