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. Author manuscript; available in PMC: 2016 Aug 2.
Published in final edited form as: Mol Pharm. 2016 Feb 1;13(3):839–851. doi: 10.1021/acs.molpharmaceut.5b00763

Figure 6.

Figure 6

Effects of CQ on OATP1B1-mediated [3H]E217G transport after prolonged treatment in HEK293-OATP1B1 cells. HEK293-OATP1B1 cells were seeded at 0.8 × 105 cells/well in 24-well plates, and cultured for 48 h prior to treatment. Cells were preincubated with CQ-free (CTL) or 25 μM CQ-containing medium for 2 h. At the end of pre-incubation, the culture medium was removed. CTL medium preincubated cells were cultured in CQ-free medium (CTL, black bar) and 25 μM CQ-preincubated cells were cultured in medium containing 1.5 μM CQ (grey bar), for indicated times up to 24 h. [3H]E217G accumulation (1 μM, 2 min) was determined at indicated time points (n = 4 in triplicate). Fold change and SE in [3H]E217G accumulation vs CTL at each indicated time points were estimated by generalized linear mixed models, as described in the Experimental Section. * indicates a statistically significant difference (p < 0.05) vs CTL.