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. Author manuscript; available in PMC: 2016 Aug 2.
Published in final edited form as: Mol Pharm. 2016 Feb 1;13(3):839–851. doi: 10.1021/acs.molpharmaceut.5b00763

Figure 8.

Figure 8

Effects of CQ on kinetic parameters of OATP1B1-mediated [3H]E217G transport. HEK293-OATP1B1 and -Mock cells were seeded at 1.1 × 105 cells/well in 24-well plates and were cultured for 48 h prior to experiment. The concentration-dependent accumulation of [3H]E217G (0.1–40 μM, 2 min) was determined in HEK293-OATP1B1 and -Mock cells pretreated with CTL or CQ (25 μM, 2 h). Values of [3H]E217G accumulation in Mock cells were subtracted from those in HEK293-OATP1B1 cells. Vmax and Km values were determined as described in the Experimental Section. Solid and dashed lines represent the best fits of the Michaelis–Menten equation to the data of CTL (black circles) and CQ (25 μM, 2 h) pretreatment (white circles), respectively. Representative graph of three independent experiments in triplicate is shown. Student's t test was conducted to compare the Vmax and Km values between CQ and CTL pretreatment. * indicates a statistically significant difference (p < 0.05; CQvs CTL).