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. 2016 Aug 2;16:567. doi: 10.1186/s12885-016-2632-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Identification of genes whose expression altered in p44 or PRMT5 silencing cells. a-d RT-PCR analysis of gene expression. Relative mRNA levels = mRNA in A549 cells expressing p44 (a, b) or PRMT5 (c, d) shRNA/mRNA in NT shRNA expressing A549 cells. e Western blot analysis of whole-cell lysates derived from NT (lanes 1 and 3), p44 (lane 2), or PRMT5 (lane 4) shRNA-expressing A549 cells with antibodies as indicated