Fig. 2.

Cortex Phellodendri antiviral activities in HEK293T and HeLa cells. HEK293T and HeLa cells treated with media alone, 1.0 μg/ml CP, or 1000 unit/ml rhIFN-β at 12 h prior to infection with a VSV-GFP or b HSV-GFP or c H3-GFP or d EV-71 at 1.0, 1.0, 3.0 and 0.5 MOI, respectively. Images were obtained at 24 hpi (200X magnification). The tests were performed in triplicate. Virus titrations are expressed as mean ± SD. Error bars indicate the range of values obtained from two independent experiments. Cell viabilities were determined by trypan blue exclusion, presented as a percentage of the control (cells without treatment). (*P < 0.05 indicates a significant difference between groups)