Table 1.

16S gene composition of the mock community

	Strain	Genome sizea	# 16S genesa	Genome copies per microgramb	Reference or DSMZ catalogue numberc
1	Campylobacter jejuni 11168	1,641,481	3	5.64E+08	[23]
2	Salmonella enterica serovar Typhimurium SL1344	4,878,012	6	1.89E+08	[24]
3	Escherichia coli mg1655	4,656,144	7	1.99E+08	[25]
4	Megasphaera elsdenii LC-1	2,474,718	7	3.74E+08	DSM 20460
5	Cloacibacillus porcorum CL-84	3,585,187	3	2.57E+08	[26]
6	Brachyspira hyodysenteriae	3,050,489	1	3.04E+08	[27]
7	Haemophilus parasuis 29755	2,224,137	2	4.17E+08	[28]
8	Bordetella bronchiseptica 1289	5,207,899	3	1.78E+08	[29]
9	Staphylococcus aureus USA300	2,872,915	5	3.22E+08	[30]
10	Bacteroides thetaiotaomicron	6,293,399	5	1.47E+08	DSM 2079
11	Faecalibacterium prausnitzii A2-165	3,080,849	3	3.01E+08	DSM 17677
12	Streptococcus parasanguinis	2,153,652	4	4.30E+08	DSM 6778
13	Parabacteroides merdae	4,431,877	3	2.09E+08	DSM 19495
14	Oscillibacter valericigenes	4,470,622	3	2.07E+08	DSM 18026
15	Desulfovibrio gigas	3,788,225	4	2.45E+08	DSM 1382
16	Lactobacillus delbrueckii subspecies bulgaricus	1,864,998	9	4.97E+08	DSM 20081
17	Coriobacterium glomerans	2,115,681	2	4.38E+08	DSM 20642
18	Oxalobacter formigenes BA-2	2,509,362	1	3.69E+08	DSM 4420
19	Roseburia hominis A2-183	3,592,125	4	2.58E+08	DSM 16839
20	Methanobrevibacter smithii d	1,704,865	1	5.43E+08	DSM 2375

^aAll except C. porcorum were calculated by the Joint Genome Institute’s Integrated Microbial Genomes Database https://img.jgi.doe.gov/cgi-bin/w/main.cgi. C. porcorum was calculated manually (BioProject PRJNA335387)

^bEstimates were calculated by URI Genomics & Sequencing Center http://cels.uri.edu/gsc/cndna.html

^cGenomic DNAs were acquired from DSMZ, the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures https://www.dsmz.de/home.html

^dThis archaeon was included as a control for non-specific amplification