. 2016 Aug 2;9:380. doi: 10.1186/s13104-016-2172-6

Table 2.

Primers used in this study

Primer name	Sequence (5′–3′)^a
i5+V3	AATGATACGGCGACCACCGAGATCTACAC ATCGTACGTATGGTAATTCAATTACCGCGGCTGCTGG
i7+V1	CAAGCAGAAGACGGCATACGAGAT AACTCTCGAGTCAGTCAGCCGAGTTTGATCMTGGCTCAG
For.seq.V3	TATGGTAATTCAATTACCGCGGCTGCTGG
Rev.seq.V1	AGTCAGTCAGCCGAGTTTGATCMTGGCTCAG
Index.V1	CTGAGCCAKGATCAAACTCGGCTGACTGACT

^aIllumina’s MiSeq adaptor sequences are in bold. Underlined portion denotes the barcode region. Barcodes used in this study are from the Schloss laboratory’s MiSeq SOP (http://www.mothur.org/wiki/MiSeq_SOP; [2]). Italics denotes the V1 or V3 16S rRNA gene primer [11]