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. 2016 Aug 1;214(3):293–308. doi: 10.1083/jcb.201605090

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© 2016 Dennis et al.

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Figure 7. — RAB38 overlaps with VARP on melanosomes and accompanies GFP-VAMP7 on departing tubules. (a–l) WT melan-Ink4a cells transiently transfected with GFP-RAB38 (green) and either VARP-HA (red, a–d), or mCh-VAMP7 (red, e–l) were analyzed 48 h later. (a–h) Cells were fixed, labeled with anti-HA (a–d), and analyzed by deconvolution immuno-FM. BF images are pseudocolored blue in merge and insets (boxed regions magnified five times). (a–d) Arrowheads show VARP-HA and GFP-RAB38 puncta adjacent to melanosomes. (e–h) Arrowheads show GFP-RAB38 and mCh-VAMP7 localized to melanosomes. (i–l) Cells were analyzed by spinning-disk confocal microscopy at ∼1 fps. (j–l) Image sequence of boxed region in i, magnified five times; elapsed time (in seconds) is indicated at the lower right. A mCh-VAMP7/GFP-RAB38–labeled structure (arrowhead) emerges from a mCh-VAMP7–labeled melanosome (arrow). Bars: (main) 10 µm; (insets and j–l) 2 µm.