Clostridium difficile Spore Germination | ||||
Germination Solution (10 mM Tris (pH 7.5), 150 mM NaCl) | ||||
100 mM Glycine + 10 mM TA | 100 mM Glycine | |||
Time (minutes) | OD585 | Calculated (nmol) | OD585 | Calculated (nmol) |
0 | 0.046 | 0.00 | 0.047 | 0.00 |
2 | 0.048 | 2.54 | 0.048 | 1.27 |
4 | 0.05 | 5.07 | 0.049 | 2.54 |
6 | 0.055 | 11.41 | 0.05 | 3.80 |
8 | 0.059 | 16.49 | 0.05 | 3.80 |
10 | 0.06 | 17.76 | 0.051 | 5.07 |
12 | 0.061 | 19.02 | 0.05 | 3.80 |
14 | 0.063 | 21.56 | 0.051 | 5.07 |