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. 2016 Jun 25;(112):54146. doi: 10.3791/54146
Clostridium difficile Spore Germination
Germination Solution (10 mM Tris (pH 7.5), 150 mM NaCl)
100 mM Glycine + 10 mM TA 100 mM Glycine 
Time (minutes) OD585 Calculated (nmol) OD585 Calculated (nmol)
0 0.046 0.00 0.047 0.00
2 0.048 2.54 0.048 1.27
4 0.05 5.07 0.049 2.54
6 0.055 11.41 0.05 3.80
8 0.059 16.49 0.05 3.80
10 0.06 17.76 0.051 5.07
12 0.061 19.02 0.05 3.80
14 0.063 21.56 0.051 5.07