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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Nat Immunol. 2016 Apr 4;17(5):538–544. doi: 10.1038/ni.3417

Figure 3.

Figure 3

AMCase-deficient mice develop S. mansoni egg–induced lung granulomas. (a) Quantitative PCR analysis of Chia1 expression in lung tissue from wild-type (n = 5) or IL-13-deficient mice (IL-13-KO) (n = 5) 7 d after intravenous injection of S. mansoni eggs. (b) Immunofluorescence of lung airway sections 7 d after intravenous egg injection. (c) Granulomatous lung pathology as measured using Giemsa stain 4 or 7 d after intravenous egg injection into wild-type (day 4 (D4), n = 8; day 7 (D7), n = 11) or AMCase-KO mice (day 4, n = 11; day 7, n = 10). (d) Lung sections from mice in c scored for fibrosis by picrosirius stain. (e) Lung sections from mice in c scored for mucus in goblet cells by PAS stain. (f) Quantification of eosinophils comprising granulomas from Giemsa-stained lung sections of mice in c. (g) Quantification of BALF cells from mice in c. Scale bars: 50 µm (b), 100 µm (c–e). Data are representative of two experiments with similar results. Error bars, s.e.m.; each data point represents a value for an individual mouse.