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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Nat Immunol. 2016 Apr 4;17(5):538–544. doi: 10.1038/ni.3417

Figure 4.

Figure 4

AMCase is critical for type 2–mediated protection against N. brasiliensis. (a) Quantification of nematodes from intestines of wild-type or AMCase-deficient (AMCase-KO) mice (n = 5 per genotype per time point) on the days after infection indicated. (b) Fecal egg counts (day 6, n = 14 mice per genotype; day 7, n = 14 per genotype; day 8, n = 13 per genotype; day 9, n = 8 per genotype; day 10, n = 8 per genotype). (c) Quantitative PCR analysis of Chia1 expression in tissues from naive wild-type mice (n = 3) shown as fold values relative to expression of the housekeeping gene Rplp2. (d) Quantitative PCR analysis of gene expression in small intestinal tissue of wild-type (n = 7) or AMCase-KO mice (n = 7) 8 d after N. brasiliensis infection, shown relative to expression in naive mice. (e) Representative PAS staining from small intestinal sections from mice in d (scale bar, 100 µm). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). Data are representative of two experiments with similar results. Error bars, s.e.m.; each data point represents a value for an individual mouse.