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Flowchart of the experimental procedures. CBD: Common bile duct; CHOL: Cholesterol; DMSO: Dimethyl sulfoxide; HYP: Hypericin; IPA: 2-propanol; PEG: Polyethylene glycol; PG: Propylene glycol; RP-HPLC: Reverse phase-high performance liquid chromatography; MALDI-TOF MS: Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry; MIX: Mixed; PIG: Pigment; RT: Room temperature.
