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. Author manuscript; available in PMC: 2016 Aug 2.
Published in final edited form as: J Am Chem Soc. 2015 Jun 24;137(26):8435–8449. doi: 10.1021/jacs.5b01493

Figure 6.

Figure 6

(a) Rate constants of ABTS (magenta) or guaiacol (black) oxidation by H2O2 catalyzed by different variants of cyt c in a 25 mM HEPES buffer at pH 7.4. The concentrations of protein, guaiacol, and H2O2 were 1 µM, 10 mM, and 5 mM, respectively. The concentrations of protein, ABTS, and H2O2 were 1 µM, 200 µM, and 1 mM, respectively. (b) The hydrogen-bonding networks that involve the heme propionate HP6 in the Lys73-ligated T78C/K79G (cyan carbons), Met80-ligated C102T25 (yellow carbons) and hydroxide-ligated WT*29 (green carbons) structures.