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. 2016 Apr 26;17(7):760–768. doi: 10.1080/15384047.2016.1178430

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Figure 1. — Both normal MBD4 protein and TruMBD4 localize to the nucleus in colorectal cancer cells. (A) Upper row: light microscopy of human colon cancer cell lines HT29 (MMR-proficient, MBD4^A10/A10), HCT116 (hMLH1^−/-, MBD4^A10/A9) and HCA7 (hMLH1^−/-, MBD4^A9/A9); Second row: Indirect immunofluorescence microscopy utilizing anti-MBD4 antibody with Alexa Fluor 594-conjugated secondary antibody (staining red); Third row: DAPI staining of nuclei; Fourth row: merge of second and third row images. (B) Western blot of colorectal cancer cell nuclear lysates for MBD4 and TruMBD4 expression. Signals were detected by an LAS-4000 luminescent image analyzer (GE Healthcare Bio-Sciences) utilizing a chemiluminescent solution.