Figure 1.

P1446A-05 strongly reduces proliferation of human melanoma cell lines in 2D and 3D culture. (A) P1446A-05 exhibits antiproliferative effects across different melanoma genotypes and phenotypes. Sensitivity ranking placed cutaneous melanoma lines ahead of uveal melanoma lines. Cells were incubated with a range of P1446A-05 concentrations for 72 hours, and cell viability was analyzed using the CellTiter-Glo luminescence assay. GI₅₀ values were calculated using nonlinear regression curve fit in GraphPad Prism 6 (error bars show 95% CI). (B) Escalating doses of P1446A-05 demonstrate strong inhibition of 3D melanoma spheroids. Mel Juso cells were cultured as spheroids in matrigel and micrographs were taken after 72 hours of drug treatment. Representative images are shown; scale bars represent 50 µm in spheroid images. Analysis of spheroid size was used to calculate % inhibition relative to control.