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. 2016 Jan 25;17(7):778–784. doi: 10.1080/15384047.2016.1139267

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Figure 3. — Intracelluar target validation. P1446A-05-mediated inhibition of CDK4 and CDK9 was confirmed via protein gel blotting for relevant phosphotargets in both CDK pathways. CDK4s phosphotarget on RB protein (ser780) becomes inhibited by 24 hours across all 3 cell lines in a dose dependent manner. CDK9s phosphotarget on RNAPII's Rpb1 (ser2) revealed better inhibition at 6 hours, and was only inhibited with the highest dose of P1446A-05 at 24 hours. The housekeeping protein GAPDH served as a loading control.