Figure 4.

TDRG1 regulates the chemosensitivity of seminoma to CDDP in vivo. (A) Representative tumors in nude mice from different groups. (B) The growth curves of transplanted tumors in nude mice (n = 6), *p < 0.05, ***p < 0.0001. (C) Representative photographs of hematoxylin and eosin (H&E) and immunohistochemistry (IHC) analysis of OCT4, PLAP and Ki-67, magnification, 200. The expression index of Ki-67 in different groups was analyzed, *p < 0.05. (D) Protein in mice xenograft tumors from different groups were collected. And the key elements in PI3K/Akt/mTOR signaling and mitochondria-mediated apoptotic pathway were measured by protein gel blotting. GAPDH served as a loading control.