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. 2016 Apr 13;30(8):2741–2754. doi: 10.1096/fj.201600261R

Figure 7.

Figure 7.

ECM1 is required for the normal function of PTHrP in vitro and ex vivo. A, B) Knockdown of ECM1 via siRNA almost abolished PTHrP-mediated inhibition of Col X expression in ATDC5 (A) and C3H10T1/2 (B) cells assayed by real-time PCR. C, D) Knockdown of ECM1 via ECM1 antibody greatly impaired PTHrP-mediated inhibition of Col X expression in ATDC5 (C) and C3H10T1/2 (D) cells detected by real-time PCR. Experiments were carried out in biologic replicates (n = 3). Rel. level, relative level; siECM1, pSuper-ECM1. Values are means ± sd. *P < 0.05 vs. PBS treatment control group; #P < 0.05 between PTHrP treatment and additional administration of pSuper-ECM1.