Figure 1.

STRA6 is expressed as an 80-kDa membrane protein in the RPE. A) Western blot using a polyclonal antibody against STRA6, showing a single band of the antibody at 80 kDa in the RPE from Het but not KO mice. Coomassie stain was used as a loading control. (Arrowhead points to the STRA6 band.) B) Immunohistochemistry on a frozen section of an eye from a Het mouse using STRA6 antibody (green) showed strong staining (arrowhead) at the basolateral membrane of the RPE. DAPI (blue) was used as counterstain of nuclei of the cells in the image. (Arrowhead points to the STRA6.) INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; PI/OS, photoreceptor inner/outer segments.