Figure 6.

Vitamin A homeostasis of the brain is affected both by the Stra6 genotype and vitamin A content of the diet. A) HPLC measurements of ROL levels in whole-brain homogenates. B) ELISA measurement of RBP4 from CSF obtained from cisterna magna puncture. C) Quantitative RT-PCR measurement of Stra6 mRNA expression in Het mice subjected to feeding with VAD and VAS diet. Gapdh was used to normalize expression to a housekeeping gene. D) Standard curve for ELISA measurements with purified recombinant human RBP4. C) Immunoblot with purified recombinant human RBP4. Letters shared between groups indicate no significant difference. Statistical significance was tested by applying a Student’s t test to each group compared with each other group. Data present values from n = 6 per genotype and dietary condition.