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Purification of chloroplastic NPP1 from leaves of the UNP1 line
| Step | Total volume (ml) | Total protein (mg) | Total activity (Ua) | Specific activity (U mg–1 protein) | Purification (fold) |
|---|---|---|---|---|---|
| Leaf extract | 400 | 183.76 | 170.68 | 0.93 | – |
| Isolated chloroplast | 10 | 1.27 | 10.86 | 8.58 | 9.24 |
| Con A–Sepharose 4B | 8.5 | 0.39 | 12.31 | 31.96 | 34.41 |
| Microcon YM-100 | 0.1 | 0.02 | 2.67 | 107.52 | 115.76 |
aμmol min–1.
