Table 3.

Glycoforms detected in rice NPP1

Peak no.a	m/zb	Δc	Estimated composition	Relative contents (%)d
2	708.259	0.003	Hex3 HexNAc1	0.00 ± 0.00e
3	733.201	−0.086	Hex1 HexNAc2 dHex1	0.00 ± 0.00e
4	749.196	−0.085	Hex2 HexNAc2	0.00 ± 0.00e
7	870.222	−0.086	Hex4 HexNAc1	0.02 ± 0.01
8	881.238	−0.086	Hex2 HexNAc2 Pent1	0.01 ± 0.00
10	911.249	−0.085	Hex3 HexNAc2	0.02 ± 0.01
11	952.276	−0.085	Hex2 HexNAc3	0.01 ± 0.02
12	1,027.296	−0.086	Hex2 HexNAc2 dHex1 Pent1	0.60 ± 0.21
13	1,032.275	−0.085	Hex5 HexNAc1	0.00 ± 0.00e
14	1,043.291	−0.086	Hex3 HexNAc2 Pent1	4.91 ± 0.57
15	1,057.307	−0.085	Hex3 HexNAc2 dHex1	0.11 ± 0.00
16	1,073.302	−0.085	Hex4 HexNAc2	0.16 ± 0.01
17	1,084.318	−0.288	Hex2 HexNAc3 Pent1	0.00 ± 0.00e
18	1,114.328	−0.086	Hex3 HexNAc3	0.01 ± 0.00
20	1,189.349	−0.085	Hex3 HexNAc2 dHex1 Pent1	26.73 ± 3.99
21	1,194.328	−0.921	Hex6 HexNAc1	0.42 ± 0.10
23	1,205.344	−0.085	Hex4 HexNAc2 Pent1	2.44 ± 0.37
25	1,230.376	−0.922	Hex2 HexNAc3 dHex1 Pent1	0.13 ± 0.09
27	1,235.355	−0.085	Hex5 HexNAc2	3.88 ± 1.16
28	1,246.371	−0.085	Hex3 HexNAc3 Pent1	1.11 ± 0.33
29	1,260.386	−0.086	Hex3 HexNAc3 dHex1	1.26 ± 0.54
32	1,356.381	−0.922	Hex7 HexNAc1	0.01 ± 0.01
33	1,367.397	−0.085	Hex5 HexNAc2 Pent1	0.58 ± 0.56
35	1,392.429	−0.085	Hex3 HexNAc3 dHex1 Pent1	5.33 ± 3.95
36	1,397.408	−0.085	Hex6 HexNAc2	10.62 ± 0.17
38	1,408.423	−0.086	Hex4 HexNAc3 Pent1	1.87 ± 0.27
39	1,422.439	−0.085	Hex4 HexNAc3 dHex1	0.02 ± 0.01
41	1,449.45	−0.085	Hex3 HexNAc4 Pent1	0.34 ± 0.24
43	1,513.455	−0.085	Hex5 HexNAc2 dHex1 Pent1	0.39 ± 0.31
44	1,518.434	−0.922	Hex8 HexNAc1	0.09 ± 0.14
45	1,554.481	−0.086	Hex4 HexNAc3 dHex1 Pent1	2.40 ± 1.83
46	1,559.46	−0.085	Hex7 HexNAc2	5.85 ± 0.79
48	1,570.476	−0.086	Hex5 HexNAc3 Pent1	0.26 ± 0.10
50	1,584.492	−0.085	Hex5 HexNAc3 dHex1	0.14 ± 0.21
51	1,595.508	−0.085	Hex3 HexNAc4 dHex1 Pent1	4.96 ± 3.78
52	1,609.524	−0.085	Hex3 HexNAc4 dHex2	0.05 ± 0.04
53	1,611.503	−0.085	Hex4 HexNAc4 Pent1	0.71 ± 0.18
58	1,700.539	−0.086	Hex4 HexNAc3 dHex2 Pent1	0.00 ± 0.00e
59	1,716.534	−0.085	Hex5 HexNAc3 dHex1 Pent1	0.23 ± 0.05
60	1,721.513	−0.085	Hex8 HexNAc2	14.71 ± 3.20
62	1,732.529	−0.085	Hex6 HexNAc3 Pent1	0.02 ± 0.01
63	1,757.561	−0.085	Hex4 HexNAc4 dHex1 Pent1	4.75 ± 0.93
65	1,773.556	−0.085	Hex5 HexNAc4 Pent1	0.26 ± 0.07
66	1,787.571	−0.086	Hex5 HexNAc4 dHex1	0.00 ± 0.00e
69	1,862.592	−0.085	Hex5 HexNAc3 dHex2 Pent1	0.00 ± 0.00e
70	1,878.587	−0.085	Hex6 HexNAc3 dHex1 Pent1	0.01 ± 0.00
71	1,883.566	−0.085	Hex9 HexNAc2	4.03 ± 0.89
72	1,903.619	−0.085	Hex4 HexNAc4 dHex2 Pent1	0.35 ± 0.10
73	1,919.614	−0.085	Hex5 HexNAc4 dHex1 Pent1	0.00 ± 0.00e
74	1,933.629	−0.085	Hex5 HexNAc4 dHex2	0.02 ± 0.02
80	2,045.619	−0.085	Hex10 HexNAc2	0.03 ± 0.02
81	2,065.672	−0.085	Hex5 HexNAc4 dHex2 Pent1	0.06 ± 0.05
82	2,079.687	−0.085	Hex5 HexNAc4 dHex3	0.06 ± 0.09
84	2,179.714	−0.086	Hex5 HexNAc6 Pent1	0.00 ± 0.00e
85	2,211.729	−0.086	Hex5 HexNAc4 dHex3 Pent1	0.00 ± 0.00e

Compositional annotations for the peaks detected in MALDI-TOF MS were achieved using KEGG Glycan and GlycoSuite online databases.

Hex, hexose; HexNAc, N-acetylhexosamine; dHex, deoxyhexose; Pent, pentose.

^aPeak number indicated in Fig. 5.

^bMass-to-charge ratio.

^cDifference from the theoretical mass value.

^dValues are represented as mean ± SD (n = 3).

^eGlycan peaks are detected in this analysis.