Table 4.

Intra-day and inter-day accuracy and precision for determination of bupropion, oxidative, and reductive metabolites in human urine with analyte specific calibration ranges^a on an AB Sciex 3200 mass spectrometer.

		Intra-assay (n = 5)			Inter-assay (n = 5)
Analyte	Nominal concentration (ng/mL)	Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)	Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)
(R)-bupropion	100	103 ± 5	103	5	103 ± 8	102	8
	500	554 ± 35	111	6	568 ± 61	114	11
	1000	1013 ± 59	101	6	1032 ± 32	103	3
(S)-bupropion	25	25.9 ± 1.5	103	6	24.3 ± 3.0	97	12
	100	103 ± 12	103	12	101 ± 14	101	14
	500	557 ± 76	111	14	552 ± 34	110	6
(R,R)-hydroxybupropionc (free)	100	85 ± 8	85	9	94 ± 10	94	11
	500	494 ± 32	99	7	490 ± 41	96	8
	2000	2024 ± 213	101	11	1997 ± 121	98	6
	5000	5094 ± 434	102	9	5159 ± 323	102	6
(S,S)- hydroxybupropionc (free)	100	95 ± 6	95	7	93 ± 4	93	4
	500	524 ± 30	105	6	492 ± 50	98	10
	2000	2083 ± 221	104	11	2039 ± 171	102	8
	5000	5192 ± 386	104	7	5185 ± 363	104	7
(1S,2S)-threohydrobupropion	250	253 ± 15	101	6	253 ± 13	101	5
	750	686 ± 39	91	6	704 ± 40	94	6
	2500	2255 ± 231	90	10	2341 ± 62	94	3
(1R,2R)-threohydrobupropion	250	242 ± 17	97	7	252 ± 6	101	2
	750	692 ± 37	92	5	703 ± 40	94	6
	2500	2274 ± 168	91	7	2318 ± 39	93	2
(1R,2S)-erythrohydrobupropion	250	257 ± 21	103	8	255 ± 22	102	9
	750	694 ± 33	93	5	699 ± 34	93	5
	2500	2214 ± 175	89	8	2359 ± 24	94	1
(1S,2R)-erythrohydrobupropion	250	255 ± 13	102	5	255 ± 11	102	4
	750	705 ± 36	94	5	704 ± 41	94	6
	2500	2264 ± 188	91	8	2340 ± 72	94	3

^aCalibration ranges are provided in Table 1.

^bMean ± standard deviation.

^cFree hydroxybupropion concentrations determined following sample preparation method 1 (Fig. 4).