Table 5.

Intra-day and inter-day accuracy and precision for determination of total^a hydroxybupropion (R,R and S,S) in human urine with analyte specific calibration ranges^b on an AB Sciex 3200 mass spectrometer.

		Intra-assay (n = 5)			Inter-assay (n = 5)
Analyte	Nominal concentration (ng/mL)	Calculated Concentration (ng/mL)c	Accuracy (%)	Precision (%)	Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)
(R,R)-hydroxybupropion	100	91.3 ± 8.2	91	9	103 ± 6	103	6
	500	517 ± 46	103	9	487 ± 43	97	9
	2000	1983 ± 147	99	7	1870 ± 73	94	4
	5000	5086 ± 350	102	7	4848 ± 129	97	3
	10,000	9907 ± 536	99	5	9056 ± 337	91	4
	15,000	15338 ± 381	102	2	14802 ± 774	99	5
(S,S)-hydroxybupropion	100	95.8 ± 13.7	96	14	93.9 ± 8.1	94	9
	500	523 ± 36	105	7	472 ± 54	94	12
	2000	2000 ± 96	100	5	1927 ± 74	96	4
	5000	5346 ± 133	107	2	4976 ± 252	100	5
	10,000	10004 ± 413	100	4	9343 ± 389	93	4
	15,000	15082 ± 818	101	5	14592 ± 570	97	4

^aTotal hydroxybupropion concentrations determined following sample preparation method 2 (Fig. 4).

^bCalibration ranges are provided in Table 1.

^cMean ± standard deviation.