Table 6.

Intra-day and inter-day accuracy and precision for determination of threohydrobupropion and erythrohydrobupropion β-d-glucuronide diastereomers in human urine with analyte specific calibration ranges^a on an AB Sciex 4000 mass spectrometer.

		Intra-assay (n = 5)			Inter-assay (n = 5)
	Nominal concentration (ng/mL)	Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)	Calculated concentration (ng/mL)b	Accuracy (%)	Precision (%)
(1S,2S) threohydrobupropion β-d-glucuronide	100	98.0 ± 6.8	98	7	98.4 ± 4.0	98	4
	250	254 ± 11	102	4	256 ± 18	102	7
	500	519 ± 48	104	9	547 ± 27	109	5
	1000	959 ± 67	96	7	973 ± 40	97	4
	2500	2604 ± 131	104	5	2563 ± 113	103	4
(1R,2R) threohydrobupropion β-d-glucuronide	100	98.1 ± 7.8	98	8	100.4 ± 5.5	100	6
	250	248 ± 16	99	6	252 ± 12	101	5
	500	495 ± 51	99	10	525 ± 30	105	6
	1000	986 ± 63	99	6	993 ± 60	99	6
	2500	2639 ± 132	106	5	2614 ± 78	105	3
(1R,2S) erythrohydrobupropion β-d-glucuronide	100	99.3 ± 5.3	99	5	98.0 ± 4.4	98	4
	250	245 ± 16	98	6	248 ± 15	99	6
	500	490 ± 42	98	9	508 ± 19	102	4
	1000	1040 ± 74	104	7	1054 ± 56	105	5
	2500	2570 ± 126	103	5	2537 ± 62	101	2

^aCalibration ranges are provided in Table 1.

^bMean ± standard deviation.