. Author manuscript; available in PMC: 2017 Aug 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 24;1027:239–253. doi: 10.1016/j.jchromb.2016.05.036

Table 7.

Stability of bupropion and metabolites in human urine.

	Stability in urine (%)^a (n = 3)
Analyte	Nominal Concentration (ng/mL)	1stfreeze-thaw	2ndfreeze-thaw	3rdfreeze-thaw
(R)-bupropion	25	101 ± 8	98 ± 5	88 ± 4
(R)-bupropion	1000	103 ± 2	98 ± 11	91 ± 3
(S)-bupropion	25	112 ± 6	99 ± 5	93 ± 11
(S)-bupropion	1000	108 ± 7	95 ± 12	90 ± 6
(R,R)-hydroxybupropion	100	96 ± 7	86 ± 10	88 ± 5
(R,R)-hydroxybupropion	15,000	105 ± 5	99 ± 5	97 ± 6
(S,S)-hydroxybupropion	100	92 ± 2	88 ± 9	88 ± 5
(S,S)-hydroxybupropion	15,000	105 ± 6	100 ± 7	97 ± 6
(1S,2S)-threohydrobupropion	250	97 ± 8	96 ± 2	88 ± 6
(1S,2S)-threohydrobupropion	2500	104 ± 4	98 ± 7	95 ± 6
(1R,2R)-threohydrobupropion	250	93 ± 7	99 ± 3	88 ± 6
(1R,2R)-threohydrobupropion	2500	103 ± 6	97 ± 7	94 ± 7
(1R,2S)-erythrohydrobupropion	250	96 ± 11	96 ± 4	87 ± 7
(1R,2S)-erythrohydrobupropion	2500	105 ± 6	98 ± 6	95 ± 7
(1S,2R)-erythrohydrobupropion	250	97 ± 9	99 ± 5	89 ± 5
(1S,2R)-erythrohydrobupropion	2500	104 ± 4	97 ± 6	94 ± 6
(1S,2S)-threohydrobupropion β-d-glucuronide	100	99 ± 10	97 ± 2	91 ± 4
(1S,2S)-threohydrobupropion β-d-glucuronide	2500	105 ± 5	100 ± 9	97 ± 7
(1 R,2R)-threohydrobupropion β-d-glucuronide	100	100 ± 11	103 ± 1	97 ± 9
(1 R,2R)-threohydrobupropion β-d-glucuronide	2500	105 ± 7	102 ± 8	97 ± 7
(1R,2S)-erythrohydrobupropion β-d-glucuronide	100	102 ± 5	104 ± 3	97 ± 5
(1R,2S)-erythrohydrobupropion β-d-glucuronide	2500	105 ± 6	100 ± 7	98 ± 7

Reported values are the percentage of the initial observed concentration (prepared freshly with no freeze thaw cycles), mean ± standard deviation, n = 3.