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. 2016 Aug 2;12(8):e1006226. doi: 10.1371/journal.pgen.1006226

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© 2016 Callender et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — ndt80Δ, (NH2188), hed1Δ ndt80Δ (NH2223), dmc1Δ hed1Δ ndt80Δ (NH2166), dmc1Δ hed1-3A ndt80Δ (NH2166::pNH302-3A²) and dmc1Δ hed1-T40A ndt80Δ (NH2166::pNH302-T40A²) and dmc1Δ hed1-T40E ndt80Δ (NH2166::pNH302-T40E²) diploids were incubated in Spo medium for seven hours. The DNA was crosslinked using psoralen and digested with XhoI. Southern blots of two-dimensional gels were probed as described in [61]. A. Two-D gels. Brackets indicate the positions of the IH and two IS JMs. Dotted lines indicate DSBs. B. Quantification of JMs from two separate experiments, one of which is shown in Panel A. Error bars indicate the range. C. Quantification of the percentage of JMs (IH + IS) in two separate experiments, one of which is shown in Panel A.