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. Author manuscript; available in PMC: 2016 Oct 16.
Published in final edited form as: Science. 2015 Oct 16;350(6258):325–328. doi: 10.1126/science.aac6948

Fig. 1. Otx-a enhancer is activated by FGF and maternal determinant GATA.

Fig. 1

(A) Expression of direct activators of Otx-a enhancer, FGF and GATA. (B) The sequence of the 69-bp Otx-a enhancer, showing the five core binding sites: three for GATA (GATA) and two for ETS (GGAA). The core binding site is defined as the 4 bp recognized by all GATA and ETS transcription factors, and they are the major sites of protein-DNA interactions (32, 33). RS Otx-a enhancer variants retain all five core binding sites with the remaining sequence (49 bp) randomized. (C) Otx-a enhancer drives expression in the a6.5 (dark green) and b6.5 (light green) lineages, beginning at gastrulation. In the tailbud stage, a6.5 cells give rise to the anterior brain (br) and palps (pal), and b6.5 cells give rise to the dorsal nerve cord (nc), dorsal epidermis (epi), and two tail muscle cells (not shown).