Table 1. Heat shock proteins and their cochaperones that were identified to associate with ERα in human cells.
| Family | Gene names | Protein names (short names) | UniProt ID | Unique peptides | Unique spectra | Sequence coverage (%)* | NcSAF | L/H ratios† | PEP‡ |
|---|---|---|---|---|---|---|---|---|---|
| Hsp70 | HSPA1A | Heat shock 70 kDa protein 1A/1B (Hsp70-1) | P08107 | 28 | 927 | 60.5 | 0.286 | 8.9 | 0 |
| HSPA2 | Heat shock-related 70 kDa protein 2 | P54652 | 12 | 38 | 37.6 | 0.012 | 8.0 | 3.2E-198 | |
| HSPA5 | 78 kDa glucose-regulated protein (Grp78) | P11021 | 35 | 266 | 51.4 | 0.080 | 10.7 | 0 | |
| HSPA6 | Heat shock 70 kDa protein 6 | P17066 | 6 | 15 | 22.9 | 0.005 | 8.0 | 1.1E-121 | |
| HSPA8 | Heat shock cognate 71 kDa protein (Hsc70) | P11142 | 25 | 810 | 60.5 | 0.248 | 11.7 | 0 | |
| HSPA9 | Stress-70 protein, mitochondrial (Grp75) | P38646 | 33 | 425 | 49.9 | 0.124 | 12.1 | 0 | |
| Hsp90 | HSP90AA1 | Heat shock protein Hsp90-alpha (Hsp90α) | P07900 | 18 | 221 | 45.4 | 0.057 | 6.7 | 3.3E-195 |
| HSP90AB1 | Heat shock protein Hsp90-beta (Hsp90β) | P08238 | 19 | 72 | 47.1 | 0.022 | 5.5 | 1.7E-144 | |
| Hsp110 | HSPH1 | Heat shock protein 105 kDa (Hsp105) | Q92598 | 25 | 74 | 34.1 | 0.016 | 24.6 | 3.7E-241 |
| HSPA4 | Heat shock 70 kDa protein 4 (HspA4) | P34932 | 38 | 154 | 54.0 | 0.032 | 4.7 | 0 | |
| HSPA4L | Heat shock 70 kDa protein 4L (HspA4L) | O95757 | 22 | 60 | 35.9 | 0.012 | 26.2 | 9.2E-163 | |
| Hsp40 | DNAJA2 | DnaJ homolog subfamily A member 2 | O60884 | 2 | 4 | 6.1 | 0.002 | 2.1 | 9.0E-22 |
| DNAJA3 | DnaJ homolog subfamily A member 3, mitochondrial | Q96EY1 | 5 | 16 | 16.9 | 0.005 | 12.7 | 1.0E-55 | |
| DNAJB1 | DnaJ homolog subfamily B member 1 | P25685 | 4 | 6 | 12.6 | 0.003 | 5.6 | 1.7E-15 | |
| DNAJB4 | DnaJ homolog subfamily B member 4 | Q9UDY4 | 2 | 4 | 5.9 | 0.002 | 4.7 | 2.6E-07 | |
| DNAJB6 | DnaJ homolog subfamily B member 6 | O75190 | 3 | 8 | 9.2 | 0.004 | 2.3 | 1.2E-12 | |
| DNAJC7 | DnaJ homolog subfamily C member 7 | Q99615 | 4 | 8 | 9.9 | 0.003 | 2.0 | 4.9E-25 | |
| DNAJC9 | DnaJ homolog subfamily C member 9 | Q8WXX5 | 10 | 21 | 35.0 | 0.013 | 14.7 | 0.068 | |
| DNAJC10 | DnaJ homolog subfamily C member 10 | Q8IXB1 | 2 | 6 | 2.8 | 0.001 | 5.9 | 1.2E-07 | |
| Small Hsps | HSPB8 | Heat shock protein beta-8 (Hsp22) | Q9UJY1 | 2 | 4 | 9.7 | 0.003 | 12.0 | 0.0035 |
| Chaperonin | HSPE1 | 10 kDa heat shock protein, mitochondrial | P61604 | 3 | 10 | 31.4 | 0.016 | 15.6 | 8.3E-27 |
| Cochaperones | STUB1 | E3 ubiquitin-protein ligase CHIP (CHIP) | Q9UNE7 | 14 | 57 | 45.5 | 0.030 | 21.6 | 1.5E-111 |
| FKBP5 | Peptidyl-prolyl cis-trans isomerase FKBP5 (FKBP51) | Q13451 | 18 | 39 | 38.5 | 0.014 | 16.5 | 8.0E-108 | |
| PTGES3 | Prostaglandin E synthase 3 (p23) | Q15185 | 4 | 12 | 15.6 | 0.012 | 171.1 | 2.0E-08 |
* Coverage of all peptide sequences matched to the identified protein sequence (%).
† Ratios of light peptides (derived from Flag-ERα-expressing cells) versus heavy peptides (derived from Flag alone-expressing cells).
‡PEP: posterior error probability.