Table 7. Metabolite additions to the E. coli biomass reaction suggested by GlobalFit to resolve FPp.
| Gene | Associated reactions | Added as biomass substrate | Added as biomass product |
|---|---|---|---|
| b0194 | PROTRS | L-Prolyl-tRNA(Pro) | TRNA(Pro) |
| b0242 | GLU5K | L-Glutamate 5-phosphate | |
| b0526 | CYSTRS | L-Cysteinyl-tRNA(Cys) | TRNA(Cys) |
| b0529 | MTHFC, MTHFD | 5-Formyltetrahydrofolate | |
| b0642 | LEUTRS | L-Leucyl-tRNA(Leu) | TRNA(Leu) |
| b0680 | GLNTRS | L-Glutaminyl-tRNA(Gln) | TRNA(Gln) |
| b0893 | SERTRS, SERTRS2 | L-Seryl-tRNA(Ser) | TRNA(Ser) |
| b0930 | ASNTRS | L-Asparaginyl-tRNA(Asn) | TRNA(Asn) |
| b1637 | TYRTRS | L-Tyrosyl-tRNA(Tyr) | TRNA(Tyr) |
| b1713 | PHETRS | L-Phenylalanyl-tRNA(Phe) | TRNA(Phe) |
| b1714 | PHETRS | L-Phenylalanyl-tRNA(Phe) | TRNA(Phe) |
| b1719 | THRTRS | L-Threonyl-tRNA(Thr) | TRNA(Thr) |
| b1866 | ASPTRS | L-Aspartyl-tRNA(Asp) | TRNA(ASP) |
| b1876 | ARGTRS | L-Arginyl-tRNA(Arg) | TRNA(ARG) |
| b1912 | PGSA120, PGSA140, PGSA141, PGSA160, PGSA161, PGSA180, PGSA181 | Phosphatidylglycerophosphate (didodecanoyl, n-C12:0) or Phosphatidylglycerophosphate (ditetradecanoyl, n-C14:0) or Phosphatidylglycerophosphate (ditetradec-7-enoyl, n-C14:1) or Phosphatidylglycerophosphate (dihexadecanoyl, n-C16:0) or Phosphatidylglycerophosphate (dihexadec-9-enoyl, n-C16:1) or Phosphatidylglycerophosphate (dioctadecanoyl, n-C18:0) or Phosphatidylglycerophosphate (dioctadec-11-enoyl, n-C18:1) | |
| b2114 | METTRS | TRNA(Met) | |
| b2514 | HISTRS | L-Histidyl-tRNA(His) | TRNA(His) |
| b2551 | GHMT2r, THFAT | 5-Formyltetrahydrofolate | |
| b2913 | PGCD | 3-Phosphohydroxypyruvate | |
| b3288 | FMETTRS | N-Formylmethionyl-tRNA | |
| b3384 | TRPTRS | L-Tryptophanyl-tRNA(Trp) | TRNA(Trp) |
| b4258 | VALTRS | L-Valyl-tRNA(Val) | TRNA(Val) |