The average ± SE for female preferences of clonal sisters and non-sisters was not due to familiarity. In each experiment, 15 females were given a choice between a familiar clonal sister (red), an unfamiliar clonal sister (yellow), or an unfamiliar non-sister (blue) after a 10-month isolation period from clonal sisters. A. Females from Comal Spring, TX, familiar clonal sisters and unfamiliar non-sisters, t(15) = 5.213, p<0.0001; B. Unfamiliar clonal sisters and unfamiliar non-sisters, t(15) = 3.362, p = 0.005; C. Familiar clonal sisters and unfamiliar clonal sisters, t(14) = 2.966, p = 0.011. Females maintain the preference for clonal sisters (regardless of familiarity) and prefer familiar clonal sisters to unfamiliar clonal sisters.