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. 2016 Mar 28;107(5):619–628. doi: 10.1111/cas.12909

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Effects of secreted SERPINI1 protein in culture supernatants (CSs) of colorectal cancer cells. (a) With Western blotting the protein levels of secreted SERPINI1 in the CSs from 15 cell lines were positively correlated with the mRNA expression levels among those cells. (b) The expression level of E‐cadherin in SW620 cells did not change with the treatment of CSs from si SERPINI1‐treated cells (lane 3 vs lane 2); however, the expression of E‐cadherin was downregulated when the cells were treated with CSs from si Control‐treated cells (lane 4 and lane 5 as duplicated experiments). EMT, epithelial–mesenchymal transition.