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. 2016 Mar 30;107(5):629–637. doi: 10.1111/cas.12911

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Establishment of soluble vascular endothelial growth factor receptor‐1 (sFlt‐1)‐ and vasohibin‐1 (VASH1)‐expressing cell lines. (a) Western blot with an anti‐vascular endothelial growth factor receptor‐1 antibody. The expression of the sFlt‐1 protein was only observed in KOC‐2S/sFlt‐1 ovarian cancer cells. (b, c) Western blots with an anti‐VASH1 antibody. The expression of the VASH1 protein was only observed in SHIN‐3/VASH1 (b) and KOC‐2S/VASH1 (c) ovarian cancer cells. Actin was used as a positive control. (d) Western blot of the culture supernatant, using an anti‐VASH1 antibody. The expression of the VASH1 protein in the culture supernatant was only observed for SHIN‐3/VASH1 and KOC‐2S/VASH1.