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. 2016 Mar 30;107(5):629–637. doi: 10.1111/cas.12911

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Influence of vasohibin‐1 (VASH1) on cell growth and the cell signaling pathway. (a) In vitro cell growth in 3D cultures of SHIN‐3/LUC and SHIN‐3/VASH1 cells, and of KOC‐2S/LUC and KOC‐2S/VASH1 cells, as observed under a light microscope. (b) No significant differences were noted in the diameter of acini between the two groups for either cell line. (c) Western blots with anti‐protein kinase B (Akt), anti‐pAkt (Ser473), anti‐ERK, and anti‐pERK (Thr202/Tyr204) antibodies. No significant difference was observed in the phosphorylated Akt or ERK levels in either of the cell lines.