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. 2016 Apr 26;107(5):666–673. doi: 10.1111/cas.12914

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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T‐5224 suppressed the migration ability of head and neck squamous cell carcinoma cells. A scratch assay was used to assess the in vitro migration ability of HSC‐3‐M3 (A) and OSC‐19 (B) cells. Areas over which the cells migrated away from the initial scratched borders were quantified using ImageJ software. The migration ability of HSC‐3‐M3 and OSC‐19 were potently suppressed in medium containing T‐5224. Data represent the mean ± SD (n = 6). **Statistically significant differences from the controls are shown (P < 0.01).