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. 2016 Apr 26;107(5):674–681. doi: 10.1111/cas.12915

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Human macrophage‐mediated antibody‐dependent cellular phagocytosis (ADCP) activity of DS‐5573a and Hu‐M30 against cancer cell lines with various level of B7‐H3 expression. ADCP activity against PKH26‐labeled target cells was measured in the presence of DS‐5573a or Hu‐M30 (effector/target = 2/1). Isotype control was also evaluated as negative control. After 3 h of incubation, each sample was stained with APC‐labeled anti‐human CD11b mAb and measured by flow cytometry. Mean ± SD of triplicates are shown.