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. 2016 Apr 7;107(5):682–689. doi: 10.1111/cas.12918

Figure 2.

Figure 2

Effect of gemcitabine (GEM) on the expression of CD20 mRNA and protein. (a) TK cells were untreated or treated with GEM (1000 ng/mL) for 24, 36 and 48 h and CD20 expression was examined by western blot at each time point. Analysis of the relative CD20 expression was performed using Image J. (b) TK cells were untreated or treated with GEM (300 ng/mL) for 48 h. They were subsequently stained with a FITC‐conjugated anti‐CD20 mAb (green) and DAPI (blue). The cells were observed under immuno‐fluorescent microscopy. (c) TK and KML‐1 cells were incubated with GEM (1000 ng/mL) for 6 and 12 h, and CD20mRNA expression was examined by qRT‐PCR. The results were quantified by the delta‐Ct method using 18S as a reference (*P < 0.01).