Table 1. Temperature Dependence of TJF Relaxation Times.
| W87F |
W87F:H43A |
|||||
|---|---|---|---|---|---|---|
| Tfinal (°C)a | τ1 (μs)b | τ2 (μs)b | intensity loss %c,d | τ1 (μs)b | τ2 (μs)b | intensity loss %c,d |
| 21e | 0.6 ± 0.3 | 1.5 ± 0.1 | 10 | 0.6 ± 0.3 | 0.8 ± 0.2 | 17 |
| 25 | 0.8 ± 0.5 | 2.1 ± 0.4 | 12 | N.D.f | N.D.f | N.D.f |
| 29e | 1.0 ± 0.5 | 2.8 ± 0.3 | 14 | 0.5 ± 0.3 | 0.9 ± 0.6 | 13 |
| 33g | 0.7 ± 0.1 | 3.1 ± 0.4 | 17 | N.D.f | 2.1 ± 1.0 | 19 |
| 38 | 0.7 ± 0.2 | 4.6 ± 0.7 | 22 | 0.7 ± 0.2 | 1.9 ± 0.2 | 24 |
| 43 | 0.8 ± 0.1 | 5.6 ± 0.8 | 26 | 0.4 ± 0.1 | 5.6 ± 1.6 | 25 |
a
Final temperature following the T-jump of 7 °C.
b
Error reported is the standard error.
c
Determined as the difference between the fluorescence intensity at time zero and the fluorescence intensity at the inflection point for each respective temperature.
d
Absolute error <1% for all measurements.
e
Temperatures 22 and 28 °C are reported here for W87F:H43A instead of 21 and 29 °C.
f
N.D. denotes not determined.
g
For W87F:H43A at 33 °C, the fast transient could not be resolved and only the recovery was fit to a monoexponential.